Reoviruses

Reoviruses possess a segmented, double-stranded RNA genome. Among the reoviruses, the rotaviruses are the most significant human pathogens. They cause diarrhea in small children and the elderly and can also produce severe sequelae in immunosuppressed patients.

Diagnosis: reovirus—isolation; rotavirus—antigen detection or electron microscopy. Isolation of this viral type in cell cultures is not a routine method.

Pathogen. The name reovirus is derived from the abbreviation for respiratory enteric orphan virus, recalling that no diseases were associated with the virus upon its discovery (hence “orphan virus”). The family Reoviridae includes, in addition to phytopathogenic and zoopathogenic strains, three genera in which human pathogens are classified:

-Coltiviruses include a large number of pathogens significant in veterinary medicine as well as the human pathogen virus that causes Colorado tick fever.

-Reoviruses in the narrower sense, with three serogroups.

-Rotaviruses, groups A to F, further subdivided into subgroups, serotypes, and electropherotypes (see below). The rotaviral genome consists of ele­ven segments of double-stranded RNA. Each segment codes for one viral protein. Some segments in other reoviruses code for two or three proteins.

Pathogenesis and clinical picture.

-Coltiviruses. Colorado tick fever usually runs a mild course with fever, myalgias, nausea, and vomiting, rarely encephalitis.

-Reoviruses. Implication of these viruses in diseases is still uncertain. It appears they are capable of infecting the respiratory and intestinal tracts of children. The fact that they are also found very frequently in asympto­matic persons makes it difficult to correlate them with specific clinical pictures.

-Rotaviruses. In the mid-seventies these viruses were recognized as diar­rhea-causing viruses in infants and small children (Fig. 8.15). They are the most frequent cause of diarrhea in children aged six months to two years. It was recently discovered that they also play a role in infections of the elderly, and above all in immunosuppressed patients (e.g., bone marrow transplant patients), and can cause severe clinical pictures in these groups. Rotaviruses enter the body per os or by droplet infection, replicate in the villi of the small intestine and cause diarrhea, potentially resulting in exsiccosis.

Diagnosis. Colorado tick fever can be diagnosed serologically. Reovirus infec­tions can be diagnosed by isolating the pathogens in cell cultures. Rotaviruses do not readily grow in cell cultures for diagnostic purposes. They can be de­tected more readily under an electron microscope or in antigen assays using commercially available solid phase tests (EIA) or passive agglutination. An elegant typing method for the different rotavirus strains involves analysis of the electrophoretic mobility of the 11 dsRNA strands of the viral genome.

Epidemiology. Humans are the sole natural reservoir of the infant pathogen rotaviruses. Generalized contamination is practically 100% when children reach school age, but carriers and reinfections are still possible despite im­munity. Diarrheal infections are among the most important causes of death in small children in developing countries; 20% of these infections are due to rotaviruses. In the temperate zone, rotaviruses are implicated in fewer indi­vidual infections; here they more frequently cause winter outbreaks in hos­pitals and homes for small children. Rotaviruses remain viable for long per­iods on objects and skin (hands!) and are therefore spread rapidly by infected persons and healthy carriers. The most effective prophylactic approach is to practice stringent hygiene.

Fig. 1 TEM image of rotaviruses in stool from an infantsuffering from diarrhea. All of the viruses in the family Reoviridae possess a double icosahedral capsid. The outer capsid has a diameter of approximately 70 nm, the inner capsid approximately 40 nm. It con­tains the segmented RNAgenome, compris­ing from 10 to 12 double-stranded subunits depending on the species.

مواضيع ذات صلة

تركيب فيروس التهاب الكبد الوبائي نوع ب الخفي

REPLICATION OF VIRUSES AN OVERVIEW

مرض الجدري Smallpox

Human Herpesvirus Types 6 And 7

Viral Nucleic Acid

Determination of Viral Load in Clinical Microbiology

الفيروسات Viruses

العاثيات الضاربة Virulent Phages

العلاج الفيروسي Virotherapy

جسيمات فيروسية Virosomes

الجسيمات الفيروسية Viroplasts

Viron