Compared to progress on many other problems in molecular biology, research on the lac operon proceeded rapidly. In part, this was due to the fact that measuring the response of the lac operon to changes in the cell’s environment was easy. Addition of lactose induces the operon a thousandfold, and the assay of β-galactosidase is particularly simple. Another important reason for the rapid progress is that many useful analogs of lactose can readily be synthesized. These analogs facilitate the assay of the lac operon proteins as well as simplify the isolation of useful mutants.

The properties of several analogs are shown in Table 1. ONPG is particularly useful as its cleavage yields galactose and orthonitrophenol, which when ionized at basic pH is bright yellow. This provides a simple and sensitive assay for β-galactosidase which requires only a measurement of the optical density after incubating enzyme and substrate. IPTG is useful in inducing the operon for two reasons. First, IPTG induces by binding directly to the repressor so that the presence of functional β-galactosidase in the cells to generate the true inducer is unnecessary. Second, IPTG is not metabolized. Therefore its use for induction does not disturb the metabolic pathways of the cell as might be the case for induction by lactose. Phenylgalactose is another useful analog. It is not an inducer, but it can be metabolized if β-galactosidase is present to hydrolyze it. Therefore only permanently derepressed cells grow on medium containing phenylgalactose as the sole carbon and energy source. Such permanently induced mutants are called constitutive and result either from defective repressor that is unable to bind to the operator or from mutant operator that is no longer recognized and bound by repressor.

Table1. Properties of Lactose Analogs

مواضيع ذات صلة

Background of the lac Operon

Mutagenesis with Chemically Synthesized DNA

Altering Cloned DNA by in vitro Mutagenesis

Footprinting, Premodification and Missing Contact Probing

Megabase Sequencing

DNA Fingerprinting—Forensics

Isolation of Rare Sequences Utilizing PCR

Polymerase Chain Reaction

Southern, Northern, and Western Transfers

Finding Clones Using Antibodies Against a Protein

Finding Clones from a Known Amino Acid Sequence

Enzymatic DNA Sequencing