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Date: 20-12-2021
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Date: 5-9-2021
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Date: 15-10-2021
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Lineweaver-Burk plot
When vo is plotted against [S], it is not always possible to determine when Vmax has been achieved because of the gradual upward slope of the hyperbolic curve at high substrate concentrations. However, if 1/vo is plotted versus 1/[S], a straight line is obtained (Fig. 1). This plot, the Lineweaver-Burk plot (also called a double-reciprocal plot) can be used to calculate Km and Vmax as well as to determine the mechanism of action of enzyme inhibitors.
Figure 1 Lineweaver-Burk plot. vo = initial reaction velocity; Vmax = maximal velocity; Km = Michaelis constant; [S] = substrate concentration.
The equation describing the Lineweaver-Burk plot is:
where the intercept on the x axis is equal to − 1/Km, and the intercept on the y axis is equal to 1/Vmax. [Note: The slope = Km/Vmax.]
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