Determination of blood glucose

 Principle:

Glucose in serum amples is determined according to the following reaction:

Normal value: 65-110 mg/dl (fasting blood glucose).

Reagents:

< >Standard glucose 100 mg% Working solution:Phosphate buffer, pH 6.5                225 mmol/l

Phenol                                                0.3 mmol/l

Amino 4-antipyrine                         8.5 mmol/l

EDTA                                                 5.0 mmol/l

Peroxidase                                         300 U/l

Glucose oxidase                               1000 U/l

Sample preparation:

-Serum or Plasma collected in sodium fluoride, EDTA or heparin.

Wave length: 505 nm.                        Measurement: against blank.

Pipette into test tubes:

          Mix, let to stand for 10 min. at 37 °C or for 20 min. at 25 °C. Measure the absorbance of Sample and Standard against blank.

Calculation:

Glucose concentation in mg/dl = A   sample / A  standard  X  100