The phenomenon of repression in the lac operon was characterized by genetic experiments. These showed that a product of the lacI gene diffused through the cell and shut off expression of the lac genes. Basically these were complementation experiments that showed that lacI acted in trans to repress. A LacI^-LacZ⁺ strain constitutively ex presses β-galactosidase in the absence or presence of inducers. The lacZ gene in such a strain is repressed if an episome is introduced which carries a good lacI gene but is deleted of the lacZ gene. The genetic structure of the resulting strain is denoted F’lacI⁺ ∆lacZ/lacI^-lacZ⁺. That is, LacI protein acts in trans to repress the chromosomal lacZ gene.

Once it was established that the LacI product repressed expression, research on the lac operon shifted to learning what repressor was; whether it was RNA or protein, and how it acted to block expression of the lac enzymes. Since the target of the repressor was a sequence that could be genetically mapped, two likely possibilities existed. The opera tor could be a region on the DNA to which repressor bound to reduce the intracellular levels of functional lac messenger. Alternatively, re pressor recognized and bound to a region on the lac messenger and reduced translation efficiency. Either of these possibilities required recognition of a specific nucleotide sequence by the lacI gene product. To many investigators, such an ability seemed most reasonable for an RNA molecule. Indeed, early results obtained with RNA and protein synthesis inhibitors led to the conclusion that it was not protein. Gilbert and Müller-Hill, however, reasoned that an RNA molecule was unlikely to possess all the regulatory properties required of the lacI product. Therefore Müller-Hill designed a simple genetic experiment that indicated that repressor contained at least some protein.

His proof was that nonsense mutations could be isolated in the lacI gene. A nonsense mutation can only be isolated in a gene that encodes a protein. The starting point was a strain containing amber mutations in both the gal and his operons. This strain was then made LacI- by selection of constitutives. Most of these mutants were missense, but a few were nonsense I- mutants. Each of the constitutives was then reverted simultaneously to Gal⁺ and His⁺. The only way a cell could perform this double reversion was to become nonsense suppressing, Su⁺, and simultaneously suppress both nonsense mutations. These nonsense-suppressing revertants were then tested for lac constitutivity,

and a few were found to have simultaneously become I⁺. Consequently, these were nonsense I mutations that were now being suppressed and were forming functional repressor that turned off expression of the lac operon in the absence of inducers. This proves that the I gene product contains protein, but it does not prove that the repressor is entirely protein. The repressor had to be purified before it could be shown to be entirely protein.

مواضيع ذات صلة

The Difficulty of Detecting Wild-Type lac Repressor

An Assay for lac Repressor

The Role of Inducer Analogs in the Study of the lac Operon

Background of the lac Operon

Mutagenesis with Chemically Synthesized DNA

Altering Cloned DNA by in vitro Mutagenesis

Footprinting, Premodification and Missing Contact Probing

Megabase Sequencing

DNA Fingerprinting—Forensics

Isolation of Rare Sequences Utilizing PCR

Polymerase Chain Reaction

Southern, Northern, and Western Transfers